ANG monoclonal antibody (M05), clone 2A7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ANG.
Immunogen
ANG (AAH54880, 25 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QDNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (74)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (39.27 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ANG expression in transfected 293T cell line by ANG monoclonal antibody (M05), clone 2A7.
Lane 1: ANG transfected lysate(16.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANG is 1 ng/ml as a capture antibody.ELISA
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Gene Info — ANG
Entrez GeneID
283GeneBank Accession#
BC054880Protein Accession#
AAH54880Gene Name
ANG
Gene Alias
ALS9, HEL168, MGC22466, MGC71966, RNASE4, RNASE5
Gene Description
angiogenin, ribonuclease, RNase A family, 5
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq
Other Designations
OTTHUMP00000164020|OTTHUMP00000164021|epididymis luminal protein 168
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Interactome
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Disease
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