ALB monoclonal antibody (M01), clone 1G12-1B3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody (M01), clone 1G12-1B3.
Lane 1: ALB transfected lysate(69.4 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of ALB transfected lysate using anti-ALB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALB MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALB is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (90.75 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ALB.
Immunogen
ALB (AAH34023, 19 a.a. ~ 609 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEGGKKLVAASQAALGL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (72); Rat (74)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (90.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody (M01), clone 1G12-1B3.
Lane 1: ALB transfected lysate(69.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of ALB transfected lysate using anti-ALB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALB MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALB is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — ALB
Entrez GeneID
213GeneBank Accession#
BC034023Protein Accession#
AAH34023Gene Name
ALB
Gene Alias
DKFZp779N1935, PRO0883, PRO0903, PRO1341
Gene Description
albumin
Gene Ontology
HyperlinkGene Summary
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq
Other Designations
cell growth inhibiting protein 42|growth-inhibiting protein 20|serum albumin
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Interactome
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Disease
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Publication Reference
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Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs.
Simeonov KP, Uppal H.
PLoS One 2014 Jun; 9(6):e100134.
Application：IS, Human, Hepatocytes.
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Hypoalbuminemia in early onset dentatorubral-pallidoluysian atrophy due to leakage of albumin in multiple organs.
Nagai S, Saito Y, Endo Y, Saito T, Sugai K, Ishiyama A, Komaki H, Nakagawa E, Sasaki M, Ito K, Saito Y, Sukigara S, Ito M, Goto YI, Ito S, Matsuoka K.
Journal of Neurology 2012 Dec; 260(5):1263.
Application：IHC-P, Human, Brain, kidney, liver .
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S-adenosylmethionine-dependent protein methylation is required for expression of selenoprotein P and gluconeogenic enzymes in HepG2 human hepatocytes.
Jackson MI, Cao J, Zeng H, Uthus E, Combs GF Jr.
The Journal of Biological Chemistry 2012 Oct; 287(43):36455.
Application：WB-Ce, Human, HepG2 cells.
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Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs.
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