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Last updated: 2014/9/15
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AFP 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000174-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-AFP full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 67.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-AFP antibody (H00000174-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000174-T01
    AFP transfected lysate.
    Western Blot
    QC Testing of H00000174-T01
    Lane 1: AFP transfected lysate ( 67.1 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 174
  • Gene Name:
  • AFP
  • Gene Alias:
  • FETA,HPAFP
  • Gene Description:
  • alpha-fetoprotein
  • Gene Summary:
  • This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000160480,alpha-1-fetoprotein,alpha-fetoglobulin
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