ADH5 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ADH5 full-length
Host
Human
Theoretical MW (kDa)
41.25
Interspecies Antigen Sequence
Mouse (93); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ADH5 antibody (H00000128-B01) by Western Blots.
SDS-PAGE Gel
ADH5 transfected lysate.
Western Blot
Lane 1: ADH5 transfected lysate ( 41.25 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ADH5
Entrez GeneID
128GeneBank Accession#
NM_000671.3Protein Accession#
NP_000662.3Gene Name
ADH5
Gene Alias
ADH-3, ADHX, FDH, GSNOR
Gene Description
alcohol dehydrogenase 5 (class III), chi polypeptide
Omim ID
103710Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq
Other Designations
alcohol dehydrogenase (class III), chi polypeptide|class III alcohol dehydrogenase 5|formaldehyde dehydrogenase|glutathione-dependent formaldehyde dehydrogenase
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Interactome
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Disease
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