ACY1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACY1 transfected lysate.
Western Blot
Lane 1: ACY1 transfected lysate ( 45.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACY1 full-length
Host
Human
Theoretical MW (kDa)
45.9
Interspecies Antigen Sequence
Mouse (85); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACY1 antibody (H00000095-B01P) by Western Blots.
SDS-PAGE Gel
ACY1 transfected lysate.
Western Blot
Lane 1: ACY1 transfected lysate ( 45.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACY1
Entrez GeneID
95GeneBank Accession#
NM_000666.1Protein Accession#
NP_000657.1Gene Name
ACY1
Gene Alias
ACY1D, ACYLASE
Gene Description
aminoacylase 1
Gene Ontology
HyperlinkGene Summary
Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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