ACP2 monoclonal antibody (M01), clone M1-4A12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.
Lane 1: ACP2 transfected lysate(48.3 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of ACP2 over-expressed 293 cell line, cotransfected with ACP2 Validated Chimera RNAi ( Cat # H00000053-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ACP2 monoclonal antibody (M01), clone M1-4A12 (Cat # H00000053-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ACP2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (72.27 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ACP2.
Immunogen
ACP2 (AAH03160, 1 a.a. ~ 423 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAGKRSGWSRAALLQLLLGVNLVVMPPTQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVYDTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQGGVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPYASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRLTEPVVPKDWQQECQLASGPADTEVIVALAVCGSILFLLIVLLLTVLFRMQAQPPGYRHVADGEDHA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Isotype
IgG2b lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (72.27 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.
Lane 1: ACP2 transfected lysate(48.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ACP2 over-expressed 293 cell line, cotransfected with ACP2 Validated Chimera RNAi ( Cat # H00000053-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ACP2 monoclonal antibody (M01), clone M1-4A12 (Cat # H00000053-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to ACP2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ACP2
Entrez GeneID
53GeneBank Accession#
BC003160Protein Accession#
AAH03160Gene Name
ACP2
Gene Alias
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Gene Description
acid phosphatase 2, lysosomal
Omim ID
171650Gene Ontology
HyperlinkGene Summary
This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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