CHOP Split FISH Probe
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Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human breast cancer (FFPE) stained with CHOP Split FISH Probe. Human breast cancer showed no CHOP gene split.
Hybridization position of the probes on the chromosome:
Hybridization position of the probes on the chromosome:
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Specification
Product Description
Labeled FISH probes for identification of gene split using Fluorescent In Situ Hybridization Technique. (Technology).
Probe 1
Name: CHOP(Texas Red)
Size: Approximately 300kb
Fluorophore: Texas Red
Location: 12q13.3Probe 2
Name: CHOP(FITC)
Size: Approximately 430kb
Fluorophore: FITC
Location: 12q13.3Probe Gap
The gap between two probes is approximately 20 kb.
Origin
Human
Source
Genomic DNA
Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome:
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Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human breast cancer (FFPE) stained with CHOP Split FISH Probe. Human breast cancer showed no CHOP gene split. -
Gene Info — DDIT3
Entrez GeneID
1649Gene Name
DDIT3
Gene Alias
CEBPZ, CHOP, CHOP10, GADD153, MGC4154
Gene Description
DNA-damage-inducible transcript 3
Omim ID
126337Gene Ontology
HyperlinkOther Designations
C/EBP homologous protein|C/EBP zeta|CCAAT/enhancer-binding protein homologous protein|growth arrest- and DNA damage-inducible
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Interactome
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Pathway
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Disease
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Publication Reference
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Dedifferentiated liposarcoma with homologous lipoblastic differentiation: expanding the spectrum to include low-grade tumours.
Jau-Yu Liau,Jen-Chieh Lee,Chen-Tu Wu,Kuan-Ting Kuo,Hsuan-Ying Huang and Cher-Wei Liang.
Histopathology 2013 Apr; 62(5):702.
Application：FISA, Human, Liposarcoma.
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Dedifferentiated liposarcoma with homologous lipoblastic differentiation: expanding the spectrum to include low-grade tumours.
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