Labeled FISH probes for identification of gene split using Fluoresecent In Situ Hybridization Technique. (Technology) The 50 uL format is now available until 2014/12/31, this format does not contain DAPI Counterstain. (Please contact us for more information.)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
DAPI Counterstain (1500 ng/mL ) 250 uL
Store at 4°C in the dark.
Hybridization position of the probes on the chromosome.
Probe 1: Size: Fluorophore: Location:
PDGFB(Texas Red) Approximately 470kb Texas Red 22q13.1
Probe 2: Size: Fluorophore: Location:
PDGFB(FITC) Approximately 610kb FITC 22q13.1
The gap between two probes is approximately 50 kb
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
PDGF, B chain,Platelet-derived growth factor, beta polypeptide (oncogene SIS),becaplermin,oncogene SIS,platelet-derived growth factor 2,platelet-derived growth factor beta,platelet-derived growth factor, B chain,v-sis platelet-derived growth factor beta p