Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology) The 50 uL format is now available until 2014/12/31, this format does not contain DAPI Counterstain. (Please contact us for more information.)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
DAPI Counterstain (1500 ng/mL ) 250 uL
Store at 4°C in the dark.
Hybridization position of the probes on the chromosome.
Probe 1: Size: Fluorophore: Location:
MDM2 Approximately 270kb Texas Red 12q14.3-q15
Probe 2: Size: Fluorophore: Location:
CEN12p Approximately 530kb FITC 12p11.22
The gap between two probes is approximately 38,700 kb.
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq