Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology) The 50 uL format is now available until 2014/12/31, this format does not contain DAPI Counterstain. (Please contact us for more information.)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
DAPI Counterstain (1500 ng/mL ) 250 uL
Store at 4°C in the dark.
Hybridization position of the probes on the chromosome.
Probe 1: Size: Fluorophore: Location:
FGFR2 Approximately 330kb Texas Red 10q26
Probe 2: Size: Fluorophore: Location:
CEN10p Approximately 640kb FITC 10p11.22
The gap between two probes is approximately 91,200 kb.
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Human breast cancer (FFPE) stained with FGFR2/CEN10p FISH Probe. Left: Human breast cancer showed no FGFR2 gene amplification. Right: Human breast cancer showed FGFR2 gene amplification (FGFR2/CEN10p ratio ≥ 2).
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq