Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology) The 50 uL format is now available until 2014/12/31, this format does not contain DAPI Counterstain. (Please contact us for more information.)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
DAPI Counterstain (1500 ng/mL ) 250 uL
Store at 4°C in the dark.
Hybridization position of the probes on the chromosome.
Probe 1: Size: Fluorophore: Location:
AKT2 Approximately 160kb Texas Red 19q13.13
Probe 2: Size: Fluorophore: Location:
CEN19p Approximately 570kb FITC 19p13.11
The gap between two probes is approximately 17,800 kb.
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta