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EIF4E(Phospho S209) & EIF4E Protein Phosphorylation Antibody Pair

  • Catalog # : DP0268
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Dual recognition immunofluorescence result.

    QC Testing of DP0268
    Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse purified polyclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. Phospho-EIF4E S209 rabbit polyclonal antibody (20 ul)
    In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol)
    2. EIF4E mouse purified polyclonal antibody (40 ug)
    In 1x PBS, pH 7.2
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Entrez GeneID:
  • 1977
  • Gene Name:
  • EIF4E
  • Gene Alias:
  • CBP,EIF4E1,EIF4EL1,EIF4F,MGC111573
  • Gene Description:
  • eukaryotic translation initiation factor 4E
  • Gene Summary:
  • All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
  • Other Designations:
  • eIF-4F 25 kDa subunit,eukaryotic translation initiation factor 4E-like 1,mRNA cap-binding protein
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