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BRAF(phospho S601) & BRAF Protein Phosphorylation Antibody Pair

  • Catalog # : DP0070
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Dual recognition immunofluorescence result.

    QC Testing of DP0070
    Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. Phospho-BRAF S601 rabbit polyclonal antibody (20 ul)
    With 0.09% sodium azide.
    2. BRAF mouse monoclonal antibody (40 ug)
    In 1x PBS, pH 7.2
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Entrez GeneID:
  • 673
  • Gene Name:
  • BRAF
  • Gene Alias:
  • B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
  • Gene Description:
  • v-raf murine sarcoma viral oncogene homolog B1
  • Gene Summary:
  • This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
  • Other Designations:
  • 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
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