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- Home > Products > Ab Pairs/Kits > Ab Pairs for PP > SMAD3(phospho S208) & SMAD3 Protein Phosphorylation Antibody Pair
SMAD3(phospho S208) & SMAD3 Protein Phosphorylation Antibody Pair
- Catalog # : DP0012
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- 1 Set
- USD $ 589
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- Specification
- Product Description:
- This protein phosphorylation antibody pair set comes with two antibodies, one against the SMAD3 protein, and the other against the specific S208 phosphorylated site of SMAD3 for use in in situ Proximity Ligation Assay. See Publication Reference below.
- Reactivity:
- Human
- Quality Control Testing:
- Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
- Supplied Product:
- Antibody pair set content:
1. Phospho-SMAD3 S208 rabbit polyclonal antibody (20 ul)
In PBS (0.09% (w/v) sodium azide)
2. SMAD3 mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.
- Storage Instruction:
- Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
- 1.
- In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.
Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J, Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.
Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
- 2.
- Direct observation of individual endogenous protein complexes in situ by proximity ligation.
Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U.
Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
- 3.
- Cytokine detection by antibody-based proximity ligation.
Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, and Fredriksson S.
Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
- 4.
- Protein detection using proximity-dependent DNA ligation assays.
Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U.
Nat Biotechnol. 2002 May;20(5):473-7.
- 5.
- Highly specific detection of phosphorylated proteins by Duolink
Mats Gullberg and Ann-Catrin Andersson
Nature Methods 6. 2009
- Application Image
- In situ Proximity Ligation Assay (Cell)
- Entrez GeneID:
- 4088
- Gene Name:
- SMAD3
- Gene Alias:
- DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396
- Gene Description:
- SMAD family member 3
- Omim ID:
- 603109
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
- Other Designations:
- MAD, mothers against decapentaplegic homolog 3,SMA- and MAD-related protein 3,SMAD, mothers against DPP homolog 3,mad homolog JV15-2,mad protein homolog,mothers against decapentaplegic homolog 3
- Related Disease
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- Graft vs Host Disease
- Head and Neck Neoplasms
- Hypersensitivity
