Pfu DNA Polymerase
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Specification
Product Description
In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, and is even up to ten-fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
Concentration
5 units/uL
Quality Control Testing
Free of detectable, non-specific nucleases.
Supplied Product
10x Pfu buffer
10x Pfu Buffer with MgSO4
25 mM MgSO4Storage Buffer
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Storage Instruction
Store at -20°C. Guaranteed stable for 6 months when properly stored.
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Applications
Polymerase Chain Reaction
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