Taq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure.
Quality Control Testing:
Free of detectable, non-specific nucleases.
Amplification of 420 bp target DNA using DNA Polymerase. M : 100 bp DNA Ladder ( Cat # D0029 ). 1 ~ 3 : Taq DNA Polymerase ( Cat # D0010 ).
10x reaction buffer
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Store at -20°C. Guaranteed stable for 6 months when properly Store.