This matched antibody pair set binds to the repeating subunits of the polyethylene glycol polymer and can be employed to detect and quantify PEGylated compounds.
Quality Control Testing:
Standard curve using LipoDox as an analyte.
Sandwich ELISA detection sensitivity ranging from 0.1 ng/ml to 30 ng/ml.
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-PEG, IgM (100 ug) Stored in 1xPBS buffer with 0.02% sodium azide. 2. Detection antibody: biotinylated mouse monoclonal anti-PEG, IgG1 (100 ug). Stored in 1xPBS buffer with 0.02% sodium azide and 50% glycerol. *Reagents are sufficient for at least 3-4 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.