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Last updated: 2014/10/27
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Interactor™ - High Throughput Interaction and Quantification Assay System

Abnova’s Interactor™ is a high throughput assay system based on time resolved fluorescence resonance energy transfer (TR-FRET). Conventional applications of TR-FRET focus primarily on GPCR second messengers and kinases. Growing interest in protein-protein and protein-nucleic acid interaction assays for follow-on screening of small molecule drugs and biological compounds represent emerging applications of TR-FRET. Furthermore, TR-FRET can be setup for robust and homogeneous sandwich ELISA assay of protein quantification without plate transfer and wash step.

The FRET principle is based on the transfer of energy between two fluorophores, a donor and an acceptor. Typically, donor and acceptor fluorophores are conjugated to distinct antibodies targeting the interacting entities. As the two entities come close enough to each other, excitation of the donor by a light source triggers an energy transfer towards the acceptor, which in turn emits specific fluorescence at a given wavelength. FRET utilizes the long-lived fluorophores combined with detection on a time resolved fluorescence basis. This allows minimization of background fluorescence interference due to compounds and proteins present in biological fluids or serum that are naturally fluorescent but are short-lived compared to the long-lived labels used.


 
Catalog # : M0009
Price: Please Inquire
 
Advantages

  • Minimal background (time resolved fluorescence)
  • Low reagent consumption
  • Miniaturized assays
  • No wash required
  • Amenable to automation
 
Applications

  • Intracellular protein-protein interaction
  • Cell surface protein-protein interaction
  • Protein-peptide interaction
  • Protein-nucleic acid interaction
  • Protein quantification
 

 

 

 
 
Assay Format & Labeling Fluorophores

  • 384-well plate
  • Sandwich assay
  • Labeling fluorophores

Donor Flurophore Europium Cryptate,
Europium Chelate
Terbium Cryptate,
Terbium Chelate
Terbium Cryptate,
Terbium Chelate
Acceptor Flurophore APC, Cy5,
AlexaFluor® 647
FITC, GFP,
Alexa Fluor® 488
Rhodamine, RPE,
Alexa Fluor® 555
Excitation Filter 340 nm 340 nm 340 nm
Emission Filter
(Donor)
620 nm 460 nm 546 nm
Emission Filter
(Acceptor)
650 nm 520 nm 570 nm
 
Time Resolved Fluorescence Measurement

 
 
General

Dimensions: 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm)
Weight: 50 lbs (22.5 kg)
Power: 130 Watts max
Read method: End point, Kinetic
Wavelength Selection: Filters / Dichroics
Detection method: Filter system: Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence
Microplate types: 1- to 384-well plates
Maximum Plate Height: Fluorescence & Luminescence: 0.89" (22.6 mm)
Temperature control: To 45°C ±0.5 °C at 37°C
Shaking: Yes
Software: Gen5™ reader control, advanced data analysis, Microsoft® Excel® export. Control through USB or serial port
Automation: Yes
 
Time-Resolved Fluorescence

Light source: Xenon Flash Lamp
Sensitivity: Europium 100 fM typical with filters (10 amol/well in 384-well plate)
Wavelength range: 200 - 700 nm (900 nm option)
 
Fluorescence Intensity

Light source: Xenon Flash Lamp
Sensitivity: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
Wavelength range: 200 - 700 nm (900 nm option)
Wavelength selection: Deep blocking bandpass filters / dichroic mirrors (Top)
Dynamic range: 5 decades
Detection system: PMT detector
Reading speed: 96-well plate: 11 seconds
384-well plate: 22 seconds
 
Fluorescence Polarization

Sensitivity: 5 mP at 1 nM fluorescein typical
Wavelength range: 300 - 700 nm (900 nm option)
 
Luminescence

Sensitivity: 10 amol ATP typical (flash)
Wavelength range: 300 - 700 nm
Dynamic range: >6 decades
 
 
Antibody & Accessory

  • Antibodies for Protein-Protein Interactions
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  • Antibody Pairs for Sandwich ELISA
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  • Antibodies for Nucleic Acid Related Proteins
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  • Antibody labeling kits for TRF assays
  • Donor Flurophore
    -Europium Labeling Kit

     

    Acceptor Flurophore
    -Allophycocyanin Labeling Kit-NH2
    -Allophycocyanin Labeling Kit-SH
    -HiLyte Fluor™ 647 Labeling Kit-NH2
    -FastLink Allophycocyanin - XL Labeling Kit
    -FastLink Atto655 Labeling Kit-NH2
    -FastLink FluoProbes® 647H Labeling Kit-NH2

     

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