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Last updated: 2014/9/22
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Protemist® DT II - High Throughput Protein Expression

Abnova presents a new generation, fully automated robotic desktop protein synthesizer, Protemist® DT II, for high throughput eykaryotic protein production. The system is developed and manufactured by CellFree Sciences Co., Ltd. It synthesizes the protein of interest using a special wheat germ lysate and a bi-layer reaction. Protemist® DT II can run two scales of translational reaction, 1.2 ml (24 well) or 6 ml (6 well). Batch affinity purification is available for affinity tagged fusion protein synthesized in 6-ml (6 well) reaction scale. This is an expert system in transcription, translation, and purification. The protocol and automation is designed for easy operation obviating the need for manual handling and production.


 
Catalog # : M0003
Price: Please Inquire
 
Features

  • Wheat germ cell-free protein synthesis
  • Start with expression vector and gene of interest
  • Transcription, translation, and purification in less than 35 hours
  • Batch affinity purification for 6-well format
  • Approximately 0.5 mg/well (6-well format) or 0.1 mg/well (24-well format) of crude protein production
  • Wide temperature control range for purification (4°C to 37°C)
  • Control, record, and data retrieval by PC
  • Disposable containers, tips, and tubes
 
Programs for protein synthesis and purification

  • 1.2-ml translation scale
  • 6-ml translation scale
  • 6-ml scale translation of GST-tagged protein and its purification using reduced glutathione buffer for elution
  • 6-ml scale translation of GST-tagged protein, and its tag removal and purification in the presence of TEV protease
  • 6-ml scale translation of GST-tagged protein, and its tag removal and purification in the presence of PreScission protease
  • 6-ml scale translation of His-tagged protein and its purification using imidazole buffer for elution
  • 6-ml scale translation of His-tagged protein, and its tag removal and purification in the presence of TEV protease
 

 

 
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Demonstrations

GST fusion protein expression and purification

Translation (6-ml format) with WEPRO 1240G at 15°C for 20hrs, purification with glutathione-sepharose followed by elution with 1 ml reduction-type glutathione


M: Size marker
C: Crude sample (3µl)
E: Eluate (2µl)

Yield/well after purification
  Purity Yield
GFP 87% 153µg
GUS 82% 166µg
DHFR 91% 172µg
p38 84% 163µg
Akt1 76% 152µg
JNK2 81% 118µg
 
His fusion protein expression and purification

Translation (6-ml format) with WEPRO 1240H at 15°C for 20hrs, purification with Ni-sepharose followed by elution with 1 ml of 500mM imidazole


M: Size marker
C: Crude sample (3µl)
E: Eluate (2µl)

Yield/well after purification
  Purity Yield
GFP 83% 224µg
GUS 88% 286µg
DHFR 81% 168µg
p38 81% 236µg
Akt1 60% 151µg
JNK2 78% 191µg
 
 
Specifications

Dimensions: 79cm(h) x 50cm(d) x 70cm(w)
Weight: 100 kg
Power: 100~240 V 50/60 Hz
Voltage Fluctuation Tolerance: ±10%
Power Consumption: 350 VA
Max. Number of Samples Per Run: 6 samples: transcription, translation and affinity purification (6 ml/sample)
24 samples: transcription, translation (1.2 ml/sample)
Processing Volumes: 100 ul to 800 ul
Reagent Unit:
  • Temperature Control Method: Peltier module
  • Temperature Setting: 4°C (Fixed)
Dispensing Volume Variation: Within ±5%, CV < 1% (in dispensing 100 µl distilled water)
Ambience for Installation: 15~30°C, humidity < 75%, dew condensation to be avoided
Operational Control & Display:
  • CPU: IntelR CeleronR M Processor (1.50 GHz)
  • HDD: 36 GB
  • Memory: 256 MB
  • OS: Windows XP (English Edition)
  • Monitor : 14.1 inch
 
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