Abnova presents Protemist^® XE, a fully-automated desktop protein synthesizer for large-scale, low cost, eukaryotic protein production. The system uses a special wheat germ lysate and a batch reaction method developed and prepared by CellFree Sciences Co., Ltd. Protemist^® XE is ideally suited for producing 10 mg to over 500 mg of protein for assay development, functional and structural analysis. A unique "Filter-and-Feed" translation method maximizes protein yield by a programmed replenishment of translation buffer and mRNA. The protocol and automation is designed for easy operation obviating the need for manual handling and production.