• Customer completes a IHC custom service form (PDF, Excel File) and send to Abnova.
• Abnova prepares slides from formalin-fixed and paraffin-embedded tissues.
• Abnova performs IHC staining either on slides without pretreatment or with pretreatment, or both.
• Abnova performs dilutions of antibody according customer's instruction or performs a serial dilution of 40, 20, 10, 5, 2.5,1.25, 0.675 ug/ml.
• Abnova offers the following slide pretreatments. Customer may choose one or more protocols for the pretreatment:
  1. Pretreatment in 1X citrate buffer (pH 6.0), microwave at 750W for 20 minutes, and subsequently cool the samples.
  2. Pretreatment in 1X Tris/EDTA buffer (pH 9.0), microwave at 750W for 20 minutes, and subsequently cool the samples.
  3. Pretreatment in HCl (2N) (pH 0.6~0.9) at room temperature for 10~20 minutes.
  4. Pretreatment in 0.1% trypsin and shake for 25 minutes at 37C.

Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.

Publish: 2009/12/7 Time: 9:06

Rotary microtome is an instrument used to cut the blocks of tissue embedded in paraffin into micro fine slices (known as sections). Microtome is an important device in microscopy preparation, allowing for the preparation of samples for observation under transmitted light or electron radiation.

Publish: 2010/6/21 Time: 1:40