Abnova provides an expanding library of more than 8,500+ immunohistochemistry (IHC) antibodies on its online catalogue. Our capability is supported by a vast collection of normal and diseased tissue samples for antibody characterization. In addition, our in-house IHC team has created panels of tissue microarray for high throughput of antibody validation, biomarker discovery, and companion diagnostic development. We are eager to learn of our customers specific requirement and to facilitate their research and product development!
Normal and Diseased Clinical Samples
Pre-made Tissue and Microarray Slides
Expanding Monoclonal Antibody Catalogue
Antibody Production Infrastructure
Rich Experience and Proven Expertise
Provide Own Clinical Samples
Provide Pre-made Tissue Slides
Antibodies (with or without Characterization)
Specific Tissue and Antibody Protocols
Previous Experimental Results
General Workflow of IHC Service
Customer completes a IHC custom service form (PDF, Excel File) and send to Abnova.
Abnova prepares slides from formalin-fixed and paraffin-embedded tissues.
Abnova performs IHC staining either on slides without pretreatment or with pretreatment, or both.
Abnova performs dilutions of antibody according customer's instruction or performs a serial dilution of 40, 20, 10, 5, 2.5, 1.25, 0.675 ug/ml.
Abnova offers the following slide pretreatments. Customer may choose one or more protocols for the pretreatment :
Pretreatment in 1X citrate buffer (pH 6.0), microwave at 750W for 20 minutes, and subsequently cool the samples.
Pretreatment in 1X Tris/EDTA buffer (pH 9.0), microwave at 750W for 20 minutes, and subsequently cool the samples.
Pretreatment in HCl (2N) (pH 0.6~0.9) at room temperature for 10~20 minutes.
Pretreatment in 0.1% trypsin and shake for 25 minutes at 37C.
Abnova will sign a confidential agreement with the customer and will not to release any experimental results to the third party without customer's permission.