Goat polyclonal antibody raised against synthetic peptide of MYD88.
Immunogen:
A synthetic peptide corresponding to internal region of human MYD88.
Sequence:
C-IKYKAMKKEFP
Host:
Goat
Theoretical MW (kDa):
33.2
Reactivity:
Human
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:16000) Sandwhich ELISA (5-10 ug/mL) Western Blot (0.03-0.1 ug/mL) Immunohistochemistry (4-6 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MYD88 polyclonal antibody (Cat # PAB6752) staining (0.03 ug/mL) of human thymus lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
MYD88 polyclonal antibody (Cat # PAB6752) (4 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained after antigen retireval at pH6 with this batch of antibody.
ELISA
Sandwich ELISA
MYD88 polyclonal antibody (Cat # PAB6752) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).