Western blot analysis of CDC2 polyclonal antibody (Cat # PAB3536) in 293 cell line lysates (35 ug/lane). CDC2 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Western Blot (Transfected lysate)
Western blot analysis of CDC2 (arrow) using rabbit CDC2 polyclonal antibody (Cat # PAB3536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies).
Flow cytometric analysis of 293 cells using CDC2 polyclonal antibody (Cat # PAB3536)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase