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EPHA4 polyclonal antibody
- カタログ番号 # : PAB3007
- 頻繁に訪問する :
- サイズ
- 価格
- 在庫あ
- 入手可能
- カート
- 100 ug
- JPY ¥ 39,000
- order now, ship in 5 days
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- この価格は、 Japanでのみ有効です。国を選択して下さい。
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Phone : +886-2-87511888
Fax : +886-2-87511166- 製品は、お客様に直接出荷されます。
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of EPHA4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHA4.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Immunofluorescence (1:100)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Eph/Ephrin Profiling in Human Breast Cancer Reveals Significant Associations between Expression Level and Clinical Outcome.
Brantley-Sieders DM, Jiang A, Sarma K, Badu-Nkansah A, Walter DL, Shyr Y, Chen J.PLoS One. 2011;6(9):e24426. Epub 2011 Sep 15.
- 2.
- Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred.
Prevost N, Woulfe D, Tanaka T, Brass LF.Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9219-24. Epub 2002 Jun 25.
- 3.
- Roles of Eph receptors and ephrins in segmental patterning.
Xu Q, Mellitzer G, Wilkinson DG.Philos Trans R Soc Lond B Biol Sci. 2000 Jul 29;355(1399):993-1002.
- 4.
- Eph receptors and ephrins: effectors of morphogenesis.
Holder N, Klein R.Development. 1999 May;126(10):2033-44.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of EPHA4 polyclonal antibody (Cat # PAB3007) in HeLa cell lysate. EPHA4 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Western Blot (Transfected lysate)
- Western blot analysis of EPHA4 (arrow) using EPHA4 polyclonal antibody (Cat # PAB3007).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA4 polyclonal antibody (Cat # PAB3007) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 2043
- Protein Accession#:
- P54764
- Gene Name:
- EPHA4
- Gene Alias:
- HEK8,SEK,TYRO1
- Gene Description:
- EPH receptor A4
- Omim ID:
- 602188
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
- Other Designations:
- OTTHUMP00000164185,TYRO1 protein tyrosine kinase,ephrin receptor EphA4,ephrin type-A receptor 4,receptor protein-tyrosine kinase HEK8,tyrosine-protein kinase receptor SEK
- Gene Pathway
