Immunohistochemistry (1:50-1:200) Immunofluorescence (1-4 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.5 (40% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate). Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with INO80B polyclonal antibody (Cat # PAB23972).
Immunohistochemical staining of human kidney with INO80B polyclonal antibody (Cat # PAB23972) shows strong cytoplasmic and nuclear positivity in cells in tubules at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with INO80B polyclonal antibody (Cat # PAB23972) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.
IES2 homolog,OTTHUMP00000160419,PAP (Pim-1 associated protein) associated protein 1,PAP-1 binding protein,high mobility group AT-hook 1-like 4,zinc finger, HIT type 4