Goat polyclonal antibody raised against synthetic peptide of CLCN2.
Immunogen:
A synthetic peptide corresponding to amino acids at internal region of human CLCN2.
Sequence:
QQLDEPVNFSDCK
Host:
Goat
Theoretical MW (kDa):
100
Reactivity:
Human
Specificity:
This antibody is expected to recognize all reported isoforms (NP_004357.3; NP_001164558.1; NP_001164559.1; NP_001164560.1).
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:64000) Western Blot (0.3-1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
CLCN2 polyclonal antibody (Cat # PAB18549) (0.3 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.