Rabbit polyclonal antibody raised against synthetic peptide of MMP2.
A synthetic peptide corresponding to amino acids 475-490 of human MMP2.
Chicken, Human, Mouse, Rat
This antibody is specific to pro and active forms of MMP-2. It does not react with other MMPs tested.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Immunohistochemistry (Frozen sections) (2-4 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL) Western Blot (1:1500-1:3500) The optimal working dilution should be determined by the end user.
In PBS (0.05% sodium azide)
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Detection of MMP2 in a Western blot of recombinant MMP2 and injured rat nerve lysate using MMP2 polyclonal antibody (Cat # PAB12382).
Western Blot (Recombinant protein)
Western blot analysis of MMP2 on recombinant proteins using MMP2 polyclonal antibody (Cat # PAB12382). MMP2 polyclonal antibody (Cat # PAB12382) is specific for MMP2 (lane 5) and does not detect recombinant mouse MMP 3 (lane 1), MMP7 (lane 2), MMP9 (Lane 3) or MMP16 (lane 4).
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
collagenase type IV-A,matrix metalloproteinase 2,matrix metalloproteinase-II,neutrophil gelatinase