The activity is determined by the dose-dependent induced proliferation of HUVECs. The expected ED50 for this effect is 5.9-8.9 ng/mL.
Storage Buffer:
Lyophilized from 10 mM NaP, pH 7.5
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of rat Vegfa, starting at 250 ng/mL, were added to HUVECs. After 87 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.