Mouse monoclonal antibody raised against full length recombinant SRC.
Immunogen:
Recombinant protein corresponding to full length human SRC.
Host:
Mouse
Reactivity:
Human, Mouse
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG1
Recommend Usage:
ELISA (1:1000) Western Blot (1:100-500) Immunohistochemistry (1:50-100) Immunoprecipitation (2 ug/test) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) is used in Western blot to detect SRC in Jurkat cell lysate.
Western Blot (Transfected lysate)
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the beta3 integrin subunit (FG-beta3) or a beta3 truncation mutant (FG-759x) . SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) was diluted 1 : 500 in 1% BSA/TBST and incubated overnight at 4 °C. After washing 3 x 5 min with TBST the blots were incubated with 1 : 5000 goat anti-mouse or goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3 x 5 min with TBST and developed using ECL reagent. Data is kindly provided by Dr. Weis of Cheresh Lab, UCSD.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq