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- Home > Products > Monoclonal Ab > Human > Binding > Nucleic Acid Binding > EIF4E monoclonal antibody, clone 5D11
EIF4E monoclonal antibody, clone 5D11
- カタログ番号 # : MAB10633
- 頻繁に訪問する :
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- 価格
- 在庫あ
- 入手可能
- カート
- 100 uL
- JPY ¥ 75,000
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- この価格は、 Japanでのみ有効です。国を選択して下さい。
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Phone : +886-2-87511888
Fax : +886-2-87511166- 製品は、お客様に直接出荷されます。
- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant EIF4E.
- Immunogen:
- Recombinant protein corresponding to human EIF4E.
- Host:
- Mouse
- Theoretical MW (kDa):
- 25
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG1
- Recommend Usage:
- ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In ascites (0.03% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) against HeLa (1) , HEK293 (2) and K-562 (3) cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical analysis of paraffin-embedded human liver cancer (A) , submaxillary tumor (B) , stomach cancer (C) and prostate cancer (D) using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) with DAB staining.
- Entrez GeneID:
- 1977
- Gene Name:
- EIF4E
- Gene Alias:
- CBP,EIF4E1,EIF4EL1,EIF4F,MGC111573
- Gene Description:
- eukaryotic translation initiation factor 4E
- Omim ID:
- 133440
- Gene Ontology:
- Hyperlink
- Gene Summary:
- All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
- Other Designations:
- eIF-4F 25 kDa subunit,eukaryotic translation initiation factor 4E-like 1,mRNA cap-binding protein
- Gene Pathway
- Related Disease
