This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of HLA-DMB transfected lysate using mouse monoclonal anti-HLA-DMB and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-HLA-DMB.
Supplied Product:
Antibody pair set content: 1. Antibody pair for IP: mouse monoclonal anti-HLA-DMB (300 ug) 2. Antibody pair for WB: rabbit polyclonal anti-HLA-DMB (50 ul)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq
Other Designations:
MHC class II HLA-DMB,MHC class II antigen HLA-DM beta chain,OTTHUMP00000029257,class II histocompatibility antigen, M beta chain