This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of CRHBP transfected lysate using mouse monoclonal anti-CRHBP and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-CRHBP.
Supplied Product:
Antibody pair set content: 1. Antibody pair for IP: mouse monoclonal anti-CRHBP (300 ug) 2. Antibody pair for WB: rabbit polyclonal anti-CRHBP (50 ul)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq