Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product:
DAPI Counterstain (1500 ng/mL ) 250 uL
Storage Instruction:
Store at 4°C in the dark.
Note:
Hybridization position of the probes on the chromosome:
Probe 1: Size: Fluorophore: Location:
NF1 Approximately 260kb Texas Red 17q11.2
Probe 2: Size: Fluorophore: Location:
CEN17q Approximately 540kb FITC 17q11.2
Probe Gap:
The gap between two probes is approximately 2,740 kb.
Origin:
Human
Source:
Genomic DNA
Notice:
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Huma lung, adenosquamous cell carcinoma (FFPE) stained with NF1/CEN17q FISH Probe. Human lung, adenosquamous cell carcinoma showed no NF1 gene amplification.
This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq