Cell freezing is the procedure to store cells for future studies. The storage of cells ensures that you have back-up cells in case of contamination or loss of cell supply. This video shows the procedure for using "Mr. Frosty". The "Mr. Frosty" is a freezing container filled with isopropyl alcohol at room temperature. It provides the idea cooling rate close to minus 1°C/minute required for successful cryopreservation of cells.