Rabbit polyclonal antibody raised against synthetic phosphopeptide of EGFR.
Synthetic phosphopeptide corresponding to residues surrounding S1070 of human EGFR.
Theoretical MW (kDa):
Human, Mouse, Rat
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis using EGFR (pS1070) polyclonal antibody (Cat # PAB10948) to detect EGFR in A-431 cell lysate, and A-431 cell treated with EGF 25 ng/ml for 15 or 30 min. Loading 50 ug total protein each well, and EGFR (pS1070) polyclonal antibody were diluted 1:1000.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor