Mouse monoclonal antibody raised against mouse Mfn1.
Immunogen:
Mouse Mfn1.
Host:
Mouse
Reactivity:
Human, Mouse
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG
Recommend Usage:
Immunocytochemistry (1:100) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris-glycine, 150mM NaCl pH 7.5 (0.05% Sodium Azide)
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MEF wild-type, Lane 2: MEF MNF1-null whole cell lysates with Mfn1 monoclonal antibody, clone 11E9-1H12 (Cat # MAB10282).
Immunohistochemical staning of mouse liver with Mfn1 monoclonal antibody, clone 11E9-1H12 (Cat # MAB10282).
Immunocytochemistry
Immunocytochemcal staining of HepG2 cells with Mfn1 monoclonal antibody, clone 11E9-1H12 (Cat # MAB10282) at 1:100with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).