EGF recombinant monoclonal antibody, clone 18B12
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EGF.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human EGF.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry image of EGF recombinant monoclonal antibody, clone 18B12 diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing SH-SY5Y cells stained with EGF recombinant monoclonal antibody, clone 18B12 (red line) at 1:50. -
Gene Info — EGF
Entrez GeneID
1950Protein Accession#
P01133Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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Pathway
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