ATP2A2 recombinant monoclonal antibody, clone 20D1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ATP2A2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ATP2A2.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemistry image of ATP2A2 recombinant monoclonal antibody, clone 20D1 diluted at 1:50 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of MCF-7 Cells with ATP2A2 recombinant monoclonal antibody, clone 20D1 at 1:25, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with ATP2A2 recombinant monoclonal antibody, clone 20D1 (red line) at 1:50. -
Gene Info — ATP2A2
Entrez GeneID
488Protein Accession#
P16615Gene Name
ATP2A2
Gene Alias
ATP2B, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, MGC45367, SERCA2
Gene Description
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2|ATPase, Ca++ transporting, slow twitch 2|SR Ca(2+)-ATPase 2|calcium pump 2|calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform|cardiac Ca2+ ATPase|endoplasmic r
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