NSDHL recombinant monoclonal antibody, clone 20F2
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: Hela whole cell lysate; Lane3: PC-3 whole cell lysate; Lane 4: HEK293 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of NSDHL recombinant monoclonal antibody, clone 20F2 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NSDHL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NSDHL.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry(1:50-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: Hela whole cell lysate; Lane3: PC-3 whole cell lysate; Lane 4: HEK293 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of NSDHL recombinant monoclonal antibody, clone 20F2 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NSDHL
Entrez GeneID
50814Protein Accession#
Q15738Gene Name
NSDHL
Gene Alias
H105E3, SDR31E1, XAP104
Gene Description
NAD(P) dependent steroid dehydrogenase-like
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000025902|short chain dehydrogenase/reductase family 31E, member 1|sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
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Interactome
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Pathway
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