NGF recombinant monoclonal antibody, clone 5F10
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More Files
- More Functions
Western Blot
Western blot analysis of U-87 whole cell lysate, Rat Brain whole cell lysate with NGF recombinant monoclonal antibody, clone 5F10 (Cat # RAB07531).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using NGF recombinant monoclonal antibody, clone 5F10 (Cat # RAB07531) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NGF.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NGF.
Theoretical MW (kDa)
Calculated MW: 27
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of U-87 whole cell lysate, Rat Brain whole cell lysate with NGF recombinant monoclonal antibody, clone 5F10 (Cat # RAB07531).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using NGF recombinant monoclonal antibody, clone 5F10 (Cat # RAB07531) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NGF
Entrez GeneID
4803Protein Accession#
P01138Gene Name
NGF
Gene Alias
Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Gene Description
nerve growth factor (beta polypeptide)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq
Other Designations
OTTHUMP00000013653|beta-nerve growth factor|nerve growth factor, beta polypeptide|nerve growth factor, beta subunit
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Interactome
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Pathway
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Disease
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