Products

Western Blot

Western blot analysis of K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate with BCL2L1 recombinant monoclonal antibody, clone 5E9 (Cat # RAB07530).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human breast cancer with BCL2L1 recombinant monoclonal antibody, clone 5E9 (Cat # RAB07530) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

598

Protein Accession#

Q07817

Gene Name

BCL2L1

Gene Alias

BCL-XL/S, BCL2L, BCLX, Bcl-X, DKFZp781P2092, bcl-xL, bcl-xS

Gene Description

BCL2-like 1

Omim ID

600039

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq

Other Designations

OTTHUMP00000030550|OTTHUMP00000030551|OTTHUMP00000030553