Products

Immunofluorescence

Immunofluorescent staining of Hela Cells with AKR1C3 recombinant monoclonal antibody, clone 4D12 (Cat # RAB07521). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometry analysis of A549 cells stained with AKR1C3 recombinant monoclonal antibody, clone 4D12 (Cat # RAB07521). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Entrez GeneID

8644

Protein Accession#

P42330

Gene Name

AKR1C3

Gene Alias

DD3, DDX, HA1753, HAKRB, HAKRe, HSD17B5, KIAA0119, hluPGFS

Gene Description

aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)

Omim ID

603966

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq

Other Designations

OTTHUMP00000018996|aldo-keto reductase family 1, member C3|chlordecone reductase|dihydrodiol dehydrogenase 3|dihydrodiol dehydrogenase X|hydroxysteroid (17-beta) dehydrogenase 5|prostaglandin F synthase|trans-1,2-dihydrobenzene-1,2-diol dehydrogenase|type