Products

Western Blot

Western blot analysis of NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse Brain whole cell lysate with CDC42 recombinant monoclonal antibody, clone 3C3 (Cat # RAB07512).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using CDC42 recombinant monoclonal antibody, clone 3C3 (Cat # RAB07512) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

998

Protein Accession#

P60953

Gene Name

CDC42

Gene Alias

CDC42Hs, G25K

Gene Description

cell division cycle 42 (GTP binding protein, 25kDa)

Omim ID

116952

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq

Other Designations

GTP-binding protein, 25kD|OTTHUMP00000002834|OTTHUMP00000002926|cell division cycle 42|cell division cycle 42 (GTP binding protein, 25kD)|cell division cycle 42 (GTP-binding protein, 25kD)|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|d