XDH recombinant monoclonal antibody, clone 8A9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate with XDH recombinant monoclonal antibody, clone 8A9 (Cat # RAB07470).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using XDH recombinant monoclonal antibody, clone 8A9 (Cat # RAB07470) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human XDH.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human XDH.
Theoretical MW (kDa)
147
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate with XDH recombinant monoclonal antibody, clone 8A9 (Cat # RAB07470).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using XDH recombinant monoclonal antibody, clone 8A9 (Cat # RAB07470) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — XDH
Entrez GeneID
7498Protein Accession#
P47989Gene Name
XDH
Gene Alias
XO, XOR
Gene Description
xanthine dehydrogenase
Gene Ontology
HyperlinkGene Summary
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq
Other Designations
OTTHUMP00000125231|xanthene dehydrogenase|xanthine oxidase|xanthine oxidoreductase
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Interactome
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Pathway
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Disease
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