VDR recombinant monoclonal antibody, clone 3C7
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human VDR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human VDR.
Theoretical MW (kDa)
Calculated MW: 49, 5
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate with VDR recombinant monoclonal antibody, clone 3C7 (Cat # RAB07432).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer using VDR recombinant monoclonal antibody, clone 3C7 (Cat # RAB07432) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VDR
Entrez GeneID
7421Protein Accession#
P11473Gene Name
VDR
Gene Alias
NR1I1
Gene Description
vitamin D (1,25- dihydroxyvitamin D3) receptor
Gene Ontology
HyperlinkGene Summary
This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
vitamin D (1,25-dihydroxyvitamin D3) receptor
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Interactome
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Disease
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