Products

Western Blot (Cell lysate)

Western blot analysis of Hela whole cell lysate, HepG2 whole cell lysate, L02 whole cell lysate with CYP1A2 recombinant monoclonal antibody, clone 8B2 (Cat # RAB07391).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human kidney tissue using CYP1A2 recombinant monoclonal antibody, clone 8B2 (Cat # RAB07391) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

1544

Protein Accession#

P05177

Gene Name

CYP1A2

Gene Alias

CP12, P3-450, P450(PA)

Gene Description

cytochrome P450, family 1, subfamily A, polypeptide 2

Omim ID

124060

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq

Other Designations

P450 form 4|aryl hydrocarbon hydroxylase|cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2|dioxin-inducible P3-450|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase