NUF2 recombinant monoclonal antibody, clone R06-9Q1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human Nuf2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human Nuf2
Theoretical MW (kDa)
Calculated MW: 54 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow cytometry (1/50-1/100)
Immunofluorescence (1/50-1/200)
Western Blot (1/500-1/1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol and 0.02% Sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — NUF2
Entrez GeneID
83540Gene Name
NUF2
Gene Alias
CDCA1, NUF2R
Gene Description
NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
Other Designations
NUF2, NDC80 kinetochore complex component|OTTHUMP00000032177|OTTHUMP00000032178|cell division cycle associated 1
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Interactome
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Disease
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