PELP1 recombinant monoclonal antibody, clone R03-8F7
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: MCF-7 whole cell lysate and Lane 3: HepG2 whole cell lysate with PELP1 recombinant monoclonal antibody, clone R03-8F7 (Cat # RAB04477).
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with PELP1 recombinant monoclonal antibody, clone R03-8F7 (Cat # RAB04477) (green). DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PELP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PELP1.
Theoretical MW (kDa)
Calculated MW: 120 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, 150mM NaCl, pH 7.4 (40% glycerol, 0.05% BSA and 0.01% Sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: MCF-7 whole cell lysate and Lane 3: HepG2 whole cell lysate with PELP1 recombinant monoclonal antibody, clone R03-8F7 (Cat # RAB04477).Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with PELP1 recombinant monoclonal antibody, clone R03-8F7 (Cat # RAB04477) (green). DAPI was stained the cell nucleus (blue).Immunofluorescence
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Gene Info — PELP1
Entrez GeneID
27043Protein Accession#
Q8IZL8Gene Name
PELP1
Gene Alias
HMX3, MNAR, P160
Gene Description
proline, glutamate and leucine rich protein 1
Omim ID
609455Gene Ontology
HyperlinkGene Summary
PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004 [PubMed 15456770]).[supplied by OMIM
Other Designations
modulator of nongenomic activity of estrogen receptor|proline and glutamic acid rich nuclear protein|proline, glutamic acid and leucine rich protein 1|proline-, glutamic acid-, leucine-rich protein 1|transcription factor HMX3
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Interactome
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Disease
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