PF4 recombinant monoclonal antibody, clone R02-1E3
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PF4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PF4.
Theoretical MW (kDa)
Calculated MW: 11 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, 150mM NaCl, pH 7.4 (40% glycerol, 0.05% BSA and 0.01% Sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of rat spleen tissue with PF4 recombinant monoclonal antibody, clone R02-1E3 (Cat # RAB04471).Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate and Lane 2: HL-60 whole cell lysate with PF4 recombinant monoclonal antibody, clone R02-1E3 (Cat # RAB04471).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
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Gene Info — PF4
Entrez GeneID
5196Protein Accession#
P02776Gene Name
PF4
Gene Alias
CXCL4, MGC138298, SCYB4
Gene Description
platelet factor 4
Omim ID
173460Gene Ontology
HyperlinkGene Summary
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM
Other Designations
chemokine (C-X-C motif) ligand 4|platelet factor 4 (chemokine (C-X-C motif) ligand 4)
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